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1

A protease substrate profiling method that links site-specific proteolysis with antibiotic resistance

Sandersjöö, Lisa, Kostallas, George, Löfblom, John, Samuelson, Patrik

Journal:

Biotechnology Journal

Year:

2014

Language:

english

File:

PDF, 1.52 MB

english, 2014

2

Investigating affinity-maturation strategies and reproducibility of fluorescence-activated cell sorting using a recombinant ADAPT library displayed on staphylococci

Åstrand, Mikael, Nilvebrant, Johan, Björnmalm, Mattias, Lindbo, Sarah, Hober, Sophia, Löfblom, John

Journal:

Protein Engineering Design and Selection

Year:

2016

Language:

english

File:

PDF, 792 KB

english, 2016

3

VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in Soluble Form or Immobilized via Spider Silk

G&uuml, ler, Rezan, Thatikonda, Naresh, Ali Ghani, Hawraa, Hedhammar, My, Löfblom, John

Journal:

ACS Biomaterials Science & Engineering

Year:

2019

File:

PDF, 1019 KB

2019

4

Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display

John Löfblom, Henrik Wernérus, Stefan Ståhl

Journal:

FEMS Microbiology Letters

Year:

2005

Language:

english

File:

PDF, 267 KB

english, 2005

5

Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase

Barbara Hjelm, Carmen Díez Fernández, John Löfblom, Stefan Ståhl, Henrik Johannesson, Johan Rockberg, Mathias Uhlén

Journal:

New Biotechnology

Year:

2010

Language:

english

File:

PDF, 784 KB

english, 2010

6

Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display

Nina Kronqvist, John Löfblom, Denise Severa, Stefan Ståhl, Henrik Wernérus

Journal:

FEMS Microbiology Letters

Year:

2008

Language:

english

File:

PDF, 573 KB

english, 2008

7

Epitope mapping of antibodies using bacterial surface display

Rockberg, Johan, Löfblom, John, Hjelm, Barbara, Uhlén, Mathias, Ståhl, Stefan

Journal:

Year:

2008

Language:

english

File:

PDF, 569 KB

english, 2008

8

Bacterial display in combinatorial protein engineering

Dr. John Löfblom

Journal:

Biotechnology Journal

Year:

2011

Language:

english

File:

PDF, 500 KB

english, 2011

9

Non-immunoglobulin based protein scaffolds

John Löfblom, Fredrik Y Frejd, Stefan Ståhl

Journal:

Current Opinion in Biotechnology

Year:

2011

Language:

english

File:

PDF, 469 KB

english, 2011

10

Optimizing Membrane Protein Overexpression in the Escherichia coli strain Lemo21(DE3)

Susan Schlegel, John Löfblom, Chiara Lee, Anna Hjelm, Mirjam Klepsch, Marc Strous, David Drew, Dirk Jan Slotboom, Jan-Willem de Gier

Journal:

Journal of Molecular Biology

Year:

2012

Language:

english

File:

PDF, 466 KB

english, 2012

11

Surface display of a single-domain antibody library on Gram-positive bacteria

Fleetwood, Filippa, Devoogdt, Nick, Pellis, Mireille, Wernery, Ulrich, Muyldermans, Serge, Ståhl, Stefan, Löfblom, John

Journal:

Cellular and Molecular Life Sciences

Year:

2013

Language:

english

File:

PDF, 801 KB

english, 2013

12

Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide

Lindberg, Hanna, Johansson, Anna, Härd, Torleif, Ståhl, Stefan, Löfblom, John

Journal:

Biotechnology Journal

Year:

2013

Language:

english

File:

PDF, 987 KB

english, 2013

13

Affinity proteins and their generation

Ståhl, Stefan, Kronqvist, Nina, Jonsson, Andreas, Löfblom, John

Journal:

Journal of Chemical Technology & Biotechnology

Year:

2013

Language:

english

File:

PDF, 300 KB

english, 2013

14

Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3

Johan Nilvebrant, Mikael Åstrand, John Löfblom…

Journal:

Cellular and Molecular Life Sciences

Year:

2013

Language:

english

File:

PDF, 767 KB

english, 2013

15

Engineering bispecificity into a single albumin-binding domain aimed for drug targeting and in vivo half-life extension

Nilvebrant, Johan, Åstrand, Mikael, Löfblom, John, Hober, Sophia

Journal:

Current Opinion in Biotechnology

Year:

2013

Language:

english

File:

PDF, 53 KB

english, 2013

16

Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin-binding domain allows for affinity purification and in vivo half-life extension

Malm, Magdalena, Bass, Tarek, Gudmundsdotter, Lindvi, Lord, Martin, Frejd, Fredrik Y., Ståhl, Stefan, Löfblom, John

Journal:

Biotechnology Journal

Year:

2014

Language:

english

File:

PDF, 736 KB

english, 2014

17

Selection of an optimal cysteine-containing peptide-based chelator for labeling of affibody molecules with 188Re

Altai, Mohamed, Honarvar, Hadis, Wållberg, Helena, Strand, Joanna, Varasteh, Zohreh, Rosestedt, Maria, Orlova, Anna, Dunås, Finn, Sandström, Mattias, Löfblom, John, Tolmachev, Vladimir, Ståhl, Stefan

Journal:

European Journal of Medicinal Chemistry

Year:

2014

Language:

english

File:

PDF, 839 KB

english, 2014

18

An engineered affibody molecule with pH-dependent binding to FcRn mediates extended circulatory half-life of a fusion protein

Seijsing, Johan, Lindborg, Malin, Höidén-Guthenberg, Ingmarie, Bönisch, Heiko, Guneriusson, Elin, Frejd, Fredrik Y., Abrahmsén, Lars, Ekblad, Caroline, Löfblom, John, Uhlén, Mathias, Gräslund, Torbjör

Journal:

Proceedings of the National Academy of Sciences

Year:

2014

Language:

english

File:

PDF, 897 KB

english, 2014

19

A new prodrug form of Affibody molecules (pro-Affibody) is selectively activated by cancer-associated proteases

Sandersjöö, Lisa, Jonsson, Andreas, Löfblom, John

Journal:

Cellular and Molecular Life Sciences

Year:

2015

Language:

english

File:

PDF, 1.17 MB

english, 2015

20

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity

Fleetwood, Filippa, Klint, Susanne, Hanze, Martin, Gunneriusson, Elin, Frejd, Fredrik Y., Ståhl, Stefan, Löfblom, John

Journal:

Scientific Reports

Year:

2014

Language:

english

File:

PDF, 1.03 MB

english, 2014

21

An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negativeE. colias well as protease-mediated secretion in OmpT-positive strains

Fleetwood, Filippa, Andersson, Ken G, Ståhl, Stefan, Löfblom, John

Journal:

Microbial Cell Factories

Year:

2014

Language:

english

File:

PDF, 1.28 MB

english, 2014

22

Staphylococcal Surface Display in Combinatorial Protein Engineering and Epitope Mapping of Antibodies

Kronqvist, Nina, Malm, Magdalena, Rockberg, Johan, Hjelm, Barbara, Uhlen, Mathias, Stahl, Stefan, Lofblom, John

Journal:

Recent Patents on Biotechnology

Year:

2010

Language:

english

File:

PDF, 638 KB

english, 2010

23

Novel affinity binders for neutralization of vascular endothelial growth factor (VEGF) signaling

Fleetwood, Filippa, Güler, Rezan, Gordon, Emma, Ståhl, Stefan, Claesson-Welsh, Lena, Löfblom, John

Journal:

Cellular and Molecular Life Sciences

Year:

2016

Language:

english

File:

PDF, 1.43 MB

english, 2016

24

A truncated and dimeric format of an Affibody library on bacteria enables FACS-mediated isolation of amyloid-beta aggregation inhibitors with subnanomolar affinity

Lindberg, Hanna, Härd, Torleif, Löfblom, John, Ståhl, Stefan

Journal:

Biotechnology Journal

Year:

2015

Language:

english

File:

PDF, 2.96 MB

english, 2015

25

Targeting HER3 using mono- and bispecific antibodies or alternative scaffolds

Malm, Magdalena, Frejd, Fredrik Y, Ståhl, Stefan, Löfblom, John

Journal:

Year:

2016

Language:

english

File:

PDF, 1.73 MB

english, 2016

26

Comparative evaluation of Affibody molecules for radionuclide imaging of in vivo expression of carbonic anhydrase IX

Garousi, Javad, Honarvar, Hadis, Andersson, Ken G, Mitran, Bogdan, Orlova, Anna, Buijs, Jos, Löfblom, John, Frejd, Fredrik Y., Tolmachev, Vladimir

Journal:

Molecular Pharmaceutics

Year:

2016

Language:

english

File:

PDF, 1.09 MB

english, 2016

27

Protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting

Sandersjöö, Lisa, Jonsson, Andreas, Löfblom, John

Journal:

Biotechnology Journal

Year:

2016

Language:

english

File:

PDF, 1.06 MB

english, 2016

28

Flow-cytometric screening of aggregation-inhibitors using a fluorescence-assisted intracellular method

Lindberg, Hanna, Sandersjöö, Lisa, Meister, Sebastian W., Uhlén, Mathias, Löfblom, John, Ståhl, Stefan

Journal:

Biotechnology Journal

Year:

2016

Language:

english

File:

PDF, 1.07 MB

english, 2016

29

AN AFFIBODY TO MONOMERIC Aβ AS A NOVEL THERAPEUTIC APPROACH FOR ALZHEIMER'S DISEASE PATHOLOGY

Boutajangout, Allal, Lindberg, Hanna, Awwad, Abdulaziz, Paul, Arun, Baitalmal, Rabaa, Gudmundsdotter, Lindvi, Wahlberg, Elisabeth, Hard, Torleif, Lofblom, John, Stahl, Stefan, Wisniewski, Thomas

Journal:

Alzheimer's & Dementia

Year:

2016

Language:

english

File:

PDF, 73 KB

english, 2016

30

Affibody-mediated PET imaging of HER3 expression in malignant tumours

Rosestedt, Maria, Andersson, Ken G., Mitran, Bogdan, Tolmachev, Vladimir, Löfblom, John, Orlova, Anna, Ståhl, Stefan

Journal:

Scientific Reports

Year:

2015

Language:

english

File:

PDF, 945 KB

english, 2015

31

In vivo evaluation of a novel format of a bivalent HER3-targeting and albumin-binding therapeutic affibody construct

Bass, Tarek Z., Rosestedt, Maria, Mitran, Bogdan, Frejd, Fredrik Y., Löfblom, John, Tolmachev, Vladimir, Ståhl, Stefan, Orlova, Anna

Journal:

Scientific Reports

Year:

2017

Language:

english

File:

PDF, 1.06 MB

english, 2017

32

Affibody Molecules in Biotechnological and Medical Applications

Ståhl, Stefan, Gräslund, Torbjörn, Eriksson Karlström, Amelie, Frejd, Fredrik Y., Nygren, Per-Åke, Löfblom, John

Journal:

Trends in Biotechnology

Year:

2017

Language:

english

File:

PDF, 3.23 MB

english, 2017

33

Insights from engineering the Affibody-Fc interaction with a computational-experimental method

Nosrati, Masoumeh, Solbak, Sara, Nordesjö, Olle, Nissbeck, Mikael, Dourado, Daniel F.A.R., Andersson, Ken G., Housaindokht, Mohammad Reza, Löfblom, John, Virtanen, Anders, Danielson, U. Helena, Flores

Journal:

Protein Engineering Design and Selection

Year:

2017

Language:

english

File:

PDF, 1.21 MB

english, 2017

34

The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule

Garousi, Javad, Andersson, Ken G., Dam, Johan H., Olsen, Birgitte B., Mitran, Bogdan, Orlova, Anna, Buijs, Jos, Ståhl, Stefan, Löfblom, John, Thisgaard, Helge, Tolmachev, Vladimir

Journal:

Scientific Reports

Year:

2017

Language:

english

File:

PDF, 1.66 MB

english, 2017

35

Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β

Wahlberg, Elisabet, Rahman, M. Mahafuzur, Lindberg, Hanna, Gunneriusson, Elin, Schmuck, Benjamin, Lendel, Christofer, Sandgren, Mats, Löfblom, John, Ståhl, Stefan, Härd, Torleif

Journal:

Scientific Reports

Year:

2017

Language:

english

File:

PDF, 3.20 MB

english, 2017

36

Staphylococcus carnosus: from starter culture to protein engineering platform

Löfblom, John, Rosenstein, Ralf, Nguyen, Minh-Thu, Ståhl, Stefan, Götz, Friedrich

Journal:

Applied Microbiology and Biotechnology

Year:

2017

Language:

english

File:

PDF, 1.40 MB

english, 2017

37

Cyclic versus noncyclic chelating scaffold for 89Zr-labeled ZEGFR:2377 affibody bioconjugates targeting epidermal growth factor receptor overexpression

Summer, Dominik, Garousi, Javad, Oroujeni, Maryam, Mitran, Bogdan, Andersson, Ken G, Vorobyeva, Anzhelika, Löfblom, John, Orlova, Anna, Tolmachev, Vladimir, Decristoforo, Clemens

Journal:

Molecular Pharmaceutics

Year:

2017

Language:

english

File:

PDF, 812 KB

english, 2017

38

Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules

Oroujeni, Maryam, Andersson, Ken G., Steinhardt, Xenia, Altai, Mohamed, Orlova, Anna, Mitran, Bogdan, Vorobyeva, Anzhelika, Garousi, Javad, Tolmachev, Vladimir, Löfblom, John

Journal:

Year:

2018

Language:

english

File:

PDF, 2.10 MB

english, 2018

39

Engineering Bispecificity into a Single Albumin-Binding Domain

Nilvebrant, Johan, Alm, Tove, Hober, Sophia, Löfblom, John, Hoheisel, Jörg D.

Journal:

Year:

2011

Language:

english

File:

PDF, 1006 KB

english, 2011

40

Cellular Effects of HER3-Specific Affibody Molecules

Göstring, Lovisa, Malm, Magdalena, Höidén-Guthenberg, Ingmarie, Frejd, Fredrik Y., Ståhl, Stefan, Löfblom, John, Gedda, Lars, Verma, Chandra

Journal:

Year:

2012

Language:

english

File:

PDF, 1.75 MB

english, 2012

41

Parallel Immunizations of Rabbits Using the Same Antigen Yield Antibodies with Similar, but Not Identical, Epitopes

Hjelm, Barbara, Forsström, Björn, Löfblom, John, Rockberg, Johan, Uhlén, Mathias, Lu, Shan

Journal:

Year:

2012

Language:

english

File:

PDF, 1.53 MB

english, 2012

42

Evaluation of the therapeutic potential of a HER3-binding affibody construct TAM-HER3 in comparison with a monoclonal antibody, seribantumab

Orlova, Anna, Bass, Tarek Z, Rinne, Sara S., Leitao, Charles Dahlsson, Rosestedt, Maria, Atterby, Christina, Gudmundsdotter, Lindvi, Frejd, Fredrik Y., Löfblom, John, Tolmachev, Vladimir, Stahl, Sefan

Journal:

Molecular Pharmaceutics

Year:

2018

Language:

english

File:

PDF, 1.22 MB

english, 2018

43

Autotransporter-mediated display of a naïve affibody library on the outer membrane of E. coli

Andersson, Ken G., Persson, Jonas, Ståhl, Stefan, Löfblom, John

Journal:

Biotechnology Journal

Year:

2018

Language:

english

File:

PDF, 632 KB

english, 2018

44

Preclinical Evaluation of [68Ga]Ga-DFO-ZEGFR:2377: A Promising Affibody-Based Probe for Noninvasive PET Imaging of EGFR Expression in Tumors

Oroujeni, Maryam, Garousi, Javad, Andersson, Ken, Löfblom, John, Mitran, Bogdan, Orlova, Anna, Tolmachev, Vladimir

Journal:

Year:

2018

Language:

english

File:

PDF, 2.18 MB

english, 2018

45

Influence of Molecular Design on the Targeting Properties of ABD-Fused Mono- and Bi-Valent Anti-HER3 Affibody Therapeutic Constructs

Altai, Mohamed, Leitao, Charles, Rinne, Sara, Vorobyeva, Anzhelika, Atterby, Christina, Ståhl, Stefan, Tolmachev, Vladimir, Löfblom, John, Orlova, Anna

Journal:

Year:

2018

Language:

english

File:

PDF, 1.58 MB

english, 2018

46

Directed evolution of the 3C protease from Coxsackievirus using a novel fluorescence-assisted intracellular method

Meister, Sebastian W., Hendrikse, Natalie M., Löfblom, John

Journal:

Biological Chemistry

Year:

2018

Language:

english

File:

PDF, 1.18 MB

english, 2018

47

Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

Rinne, Sara S., Leitao, Charles Dahlsson, Mitran, Bogdan, Bass, Tarek Z., Andersson, Ken G., Tolmachev, Vladimir, Ståhl, Stefan, Löfblom, John, Orlova, Anna

Journal:

Scientific Reports

Year:

2019

File:

PDF, 1.59 MB

2019

48

Comparative evaluation of two DARPin variants: effect of affinity, size and label on tumor targeting properties

Deyev, Sergey Mikhailovich, Vorobyeva, Anzhelika, Schulga, Alexey, Proshkina, Galina, Güler, Rezan, Löfblom, John, Mitran, Bogdan, Garousi, Javad, Altai, Mohamed, Buijs, Jos, Orlova, Anna, Tolmachev,

Journal:

Molecular Pharmaceutics

Year:

2019

Language:

english

File:

PDF, 911 KB

english, 2019

49

Molecular Design of HER3-Targeting Affibody Molecules: Influence of Chelator and Presence of HEHEHE-Tag on Biodistribution of 68Ga-Labeled Tracers

Dahlsson Leitao, Charles, Rinne, Sara, Mitran, Bogdan, Vorobyeva, Anzhelika, Andersson, Ken, Tolmachev, Vladimir, Ståhl, Stefan, Löfblom, John, Orlova, Anna

Journal:

International Journal of Molecular Sciences

Year:

2019

File:

PDF, 1.69 MB

2019

50

VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in Soluble Form or Immobilized via Spider Silk

G&uuml, ler, Rezan, Thatikonda, Naresh, Ali Ghani, Hawraa, Hedhammar, My, Löfblom, John

Journal:

ACS Biomaterials Science & Engineering

Year:

2019

Language:

english

File:

PDF, 1019 KB

english, 2019